Mapping QTL for Agronomic Traits on Wheat Chromosome 3A and a Comparison of Recombinant Inbred Chromosome Line Populations

Variation for wheat (Triticum aestivum L.) grain yield and agronomic traits was used to map quantitative trait loci (QTL) in a ‘Cheyenne’ (CNN) × [CNN (‘Wichita’ 3A)] recombinant inbred chromosome line (RICL) population consisting of 223 CNN(RICLs3A) and 7 check cultivars that were evaluated in six environments in Nebraska during 2005–2007. A chromosome 3A linkage map spanning 106 cM was constructed using 32 microsatellite markers. Composite interval mapping detected 19 QTL for seven agronomic traits that individually accounted for 4.6 to 16.8% of the phenotypic variation. Three small genomic segments, spanning 3.4, 5.3, and 5.3 cM, contained most of the QTL. Two yield QTL were detected in two environments and in data pooled over environments. For grain volume weight, a QTL was detected in fi ve of the six environments while a plant height QTL was detected in all environments. Wichita (WI) alleles contributed to the increased trait values for yield, spikes per square meter, and grain volume weight, while CNN contributed alleles to the increased 1000-kernel weight, plant height, and anthesis date. Both CNN and WI contained alleles for increased number of kernels per spike. The 223 CNN(RICLs3A) set had greater power to detect QTL than the two smaller subsets—128 CNN(RICLs3A) developed using doubled haploids and 95 CNN(RICLs3A) developed using recombinant monosomic lines. Neither of the subsets performed consistently better than the other in detecting QTL. M.L. Ali, Univ. of Arkansas, Rice Res. and Ext. Cent., 2900 Highway 130E, Stuttgart, AR 72160; P.S. Baenziger and Ismail Dweikat, Dep. of Agron. and Hortic., K.M. Eskridge, Dep. of Statistics, Univ. of Nebraska, Lincoln, NE 68583-0915; Z. Al-Ajlouni, Dep. of Plant Prod., Jordan Univ. of Sci. and Technol., Irbid-Jordan, Postal Code 22110, Jordan; B.T. Campbell, USDA-ARS, Costal Plains Res. Cent., 2611 W. Lucas St., Florence, SC 29501; K.S. Gill, Dep. of Crop and Soil Sci., Washington State Univ., Pullman, WA 99164; A. Mujeeb-Kazi, Natl. Inst. of Biotechnol. & Genet. Eng. (NIBGE), Faisalabad, Pakistan. Received 20 June 2010. *Corresponding author (pbaenziger1@ unl.edu). Abbreviations: AD, anthesis date; CNN, Cheyenne; DH, doubled haploid; GEI, genotype × environment interaction; GVWT, grain volume weight; GYLD, grain yield; KPS, kernels per spike; PHT, plant height; QTL, quantitative trait loci; RFLPs, restriction fragment length polymorphisms; RICLs, recombinant inbred chromosome lines; RMLs, recombinant monosomic lines; SPSM, spikes per square meter; SSRs, simple sequence repeats; STM, sequence-tagged microsatellite; TKWT, 1000-kernel weight; WI, Wichita. Published in Crop Sci. 51:553–566 (2011). doi: 10.2135/cropsci2010.06.0359 Published online 10 Jan. 2010. © Crop Science Society of America | 5585 Guilford Rd., Madison, WI 53711 USA All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.